Description
Biological activity
Background information
Macrophage-colony stimulating factor (M-CSF), a four α-helical bundle cytokine, is a potent hematopoietic regulator. It is primarily produced by monocytes, granulocytes, endothelial cells, and fibroblasts. The main function of M-CSF is the regulation of proliferation, differentiation, and survival of monocytes, macrophages and their hematopoietic progenitors. Furthermore, M-CSF has been shown to play an important role in immunological defense, bone metabolism, fertility, and pregnancy.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).
Overview
Recombinant human M-CSF can regulate the proliferation, differentiation, and survival of monocytes, macrophages, osteoclasts and their hematopoietic progenitors. The macrophage colony-stimulating factor (M-CSF) is a potent hematopoietic cytokine that is involved in diverse processes, such as regulation of inflammatory responses, bone resorption, atherosclerosis or brain and placental development. M-CSF recombinant protein can be especially used in differentiation studies, cell culture and functional assays.
