Miltenyi, 130-096-495, CD8+ T Cell Isolation Kit, human

Detailed separation procedure
Non-CD8+ cells, i.e., CD4+ T cells, monocytes, neutrophils, eosinophils, B cells, stem cells, dendritic cells, NK cells, granulocytes, γ/δ T cells, or erythroid cells are labeled by using a cocktail of biotin-conjugated antibodies. The cocktail contains antibodies against CD4, CD15, CD16, CD19, CD34, CD36, CD56, CD123, TCRγ/δ, and CD235a (Glycophorin A). Subsequently, non-target cells are magnetically labelled with the CD8+ T Cell MicroBead Cocktail. Isolation of highly pure T cells is achieved by depletion of magnetically labelled cells.

Downstream applications
The CD8+ T Cell Isolation Kit is the tool of choice when direct labeling of CD8+ T cell surface molecules could interfere with downstream applications, e.g., in studies on cytotoxic T cell activation1. Untouched CD8+ T cells may also be used for the isolation of specific cytotoxic T cell subsets, such as naive or memory CD8+ T cells and activated CD8+ T cells.

Columns
LS or autoMACS® Columns.

Overview
The CD8+ T Cell Isolation Kit has been developed for fast isolation of untouched cytotoxic CD8+ T cells from human peripheral blood mononuclear cells (PBMCs). This updated kit offers even better performance and a significantly shorter protocol and replaces the previous kit (#130-094-156).