Description
Background information
BKV is a ubiquitous polyomavirus. After primary infection BKV persists in a latent state. Virus reactivation might occur in immunodeficiency or during immunosuppression. The ST antigen is expressed early in the lytic cycle.
Downstream applications
PepTivator BKV ST - research grade has been specifically developed for efficient in vitro stimulation of BKV ST–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications, including:
- Detection and analysis of BKV ST–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
- Isolation of viable BKV ST–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of BKV ST–specific effector/memory T cells from naive T cell populations.
- Pulsing of antigen-presenting cells.
Overview
PepTivator® BKV ST is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the BK virus (BKV) small T (ST) antigen (UniProt ID: P15000).
The in vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers. These cytokines or activation markers then allow the detection and isolation of antigen-specific T cells.
