Miltenyi, 130-096-513, NK1.1+ iNKT Cell Isolation Kit, mouse

Background information
Natural killer T (NKT) cells are a heterogeneous group of lymphocytes that share properties of both T cells and natural killer (NK) cells. They are an important link between the innate and the adaptive immune system, promoting or suppressing immune responses.
In C57BL/6 mice, NKT cells were first defined according to their expression of the NK cell-associated marker NK1.1 (CD161). The term iNKT cells preferentially refers to CD1d-restricted T cells, expressing a heavily biased, semiinvariant T cell receptor (TCR Vα14-Jα18, Vs8.2, Vs7 and Vs2).

Detailed separation procedure
The isolation of NK1.1+ NKT cells is performed in a two-step procedure. First, non-target cells like NK cells, B cells, Macrophages, CD8+ T cells and TCRγδ+ T cells are magnetically labeled and depleted using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Subsequently, NK1.1+ iNKT cells are positively selected from the pre-enriched fraction using Anti-NK1.1-APC and Anti-APC MicroBeads.

Columns
For the first magnetic separation (depletion): LD or autoMACS® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Overview
The NK1.1+ iNKT Cell Isolation Kit was developed for the isolation of NK1.1-expressing NKT cells from murine lymphoid tissues by sequential sorting.