Miltenyi, 130-096-537, Pan Monocyte Isolation Kit, human

Detailed separation procedure
Non-monocytes, such as T cells, NK cells, B cells, dendritic cells, and basophils are indirectly magnetically labeled using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads.

Downstream applications

- Functional studies on monocytes, in which any effect due to antibody binding to target cells should be avoided.
- Studies on monocyte activation, differentiation, cytokine secretion etc.
- Studies on signal transduction in monocytes.
- Studies on antigen uptake (e.g. phagocytosis) and antigen presentation by monocytes.
- In vitro differentiation of blood monocytes into dendritic cells.

Columns
MS, LS, XS, or autoMACS® Columns.

Overview
The Pan Monocyte Isolation Kit is an indirect magnetic labeling system for the isolation of untouched monocytes from human PBMCs. This Kit allows the simultaneous enrichment of classical (CD14++CD16–), non classical (CD14+CD16++) and intermediate (CD14++CD16+) monocytes.1 Highly pure unlabeled monocytes are obtained by depletion of the magnetically labeled cells.