Description
Background information
A. fumigatus is a common saprophytic fungus. It can cause allergic bronchopulmonary aspergillosis or can induce severe infections of the respiratory tract in immunocompromised individuals. It is suggested that CD4+ T cells play a central role in the prevention and elimination of these infections.
Downstream applications
PepTivator A. fumigatus pmp20 – research grade has been specifically developed for efficient in vitro stimulation of A. fumigatus pmp20-specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications, including:
- Detection and analysis of pmp20-specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
- Isolation of viable pmp20-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of pmp20-specific effector/memory T cells from naive T cell populations.
- Pulsing of antigen-presenting cells.
Overview
PepTivator® A. fumigatus pmp20 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the Aspergillus fumigatus pmp20 antigen (UniProt ID: O43099).
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.
