Description
Background information
C. albicans is a common fungus causing opportunistic mucosal infections. The 65-kDa mannoprotein MP65 from the cell wall of C. albicans was described as a major T cell antigen and discussed to induce protective immunity against a Candida infection.
Downstream applications
PepTivator C. albicans MP65 – research grade has been specifically developed for efficient in vitro stimulation of C. albicans MP65–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications, including:
- Detection and analysis of MP65–specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
- Isolation of viable MP65–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of MP65–specific effector/memory T cells from naive T cell populations.
- Pulsing of antigen-presenting cells.
Overview
PepTivator® C. albicans MP65 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the Candida albicans MP65 antigen (UniProt ID: Q9HEP1).
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.
