Description
Background information
Anti-Biotin MicroBeads UltraPure have been especially developed for highly efficient separation of cells from debris-rich starting material. The biotinylated molecule is recognized by a monoclonal anti-biotin antibody coupled to MicroBeads. Anti-Biotin MicroBeads and Anti-Biotin MicroBeads UltraPure have the advantage of not binding to free biotin, which is often present in culture media. This feature provides the most sensitive labeling and separation of cells. With UltraPure MicroBeads we offer an ideal solution for ultrapure cell separation of cells even from debris-rich samples or other low frequency biological starting materials.
Applications
Anti-Biotin MicroBeads are ideal for positive selection, even of cells with low antigen expression, or for depletion. Anti-Biotin MicroBeads have been used for the separation of murine αE-CD25+ and αE-CD25- T cell subsets1 and for the isolation of CD45.2+ cells from the bone marrow of chimeric mice2. They have also been used for the enrichment of human CD94+ NK cells3. Anti-Biotin MicroBeads UltraPure have been especially designed for high-efficient cell isolation even from debris-rich samples and starting materials with increased amount of dead cells.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.
Overview
Anti-Biotin MicroBeads have been developed for indirect magnetic labeling and separation of cells or other biological materials that are labeled with a biotinylated primary antibody or ligand, or with a cocktail of biotinylated antibodies. When working with debris-rich starting material and low frequency samples we recommend Anti-Biotin MicroBeads UltraPure to benefit from high specific enrichment with low background of debris and dead cells.
