Description
Background information
CD133, formerly known as AC133, recognizes epitope 1 of the CD133 antigen.2,3 It is a marker that is frequently found on multipotent progenitor cells, including immature hematopoietic stem and progenitor cells. In the hematopoietic system, CD133 is expressed on a small portion of CD34– cells4 as well as on a subset of CD34bright stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood5. CD133 has also been found to be expressed on circulating endothelial progenitor cells,6,7 fetal neural stem cells,8,9 other tissue-specific stem cells, such as renal10, prostate11, and corneal12 stem cells, cancer stem cells from tumor tissues, as well as ES and iPS cell-derived cells.
There is a growing interest in CD133-expressing stem cells from normal blood or bone marrow in regenerative medicine, for example, in the field of cardiovascular13-17, liver, or peripheral artery diseases18-20.
The CD133 MicroBeads included in the kit recognize epitope CD133/1. For quality control staining of isolated CD133+ cells, the use of CD133/2 (293C3)-PE or -APC is recommended.
Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.
Overview
The CD133 MicroBead Kit allows basic isolation or depletion of CD133+ cells from various sources.
Note: Optimized isolation kits with improved performance are available for the following tissues:
- For hematopoietic or endothelial progenitor cells, choose the CD133 MicroBead Kit – Hematopoietic Tissue
- For cancer or tissue stem cells the CD133 MicroBead Kit – Tumor Tissue is recommended
