Miltenyi, 130-097-148, CD43 (Ly-48) MicroBeads, mouse - lyophilized

Background information
The CD43 antigen is expressed on nearly all leukocytes except immature and mature resting B cells.

Detailed separation procedure
By using CD43 MicroBeads, all CD43-expressing cells are magnetically labeled, whereas the resting B cell subset stays untouched and is collected in the column effluent.

Downstream applications
Resting B cells isolated with CD43 MicroBeads have been used for various studies on B cell differentiation and related molecular processes. Resting B cells isolated from spleen were used for in vitro analyses on antibody class switching.1 They were separated for comparative analysis on B cell receptor signal transduction in wild type and immunodeficient mice2 or were used as starting material for studies on germinal center formation.3

Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.

Overview
CD43 (Ly-48) MicroBeads were developed for the isolation of untouched resting B cells (B-2 cells) from peripheral lymphoid tissues or late B cell progenitors from bone marrow.