Description
Background information
Multiple sclerosis is an autoimmune disease characterized by a chronic inflammatory response against myelin sheaths around the axons in the central nervous system. The myelin sheaths are destroyed in the course of the disease. Autoreactive T cells specific for myelin antigens, such as myelin proteolipid protein (PLP), are considered to be involved in the pathogenesis of the disease.
Downstream applications
PepTivator PLP – research grade has been specifically developed for efficient in vitro stimulation of PLP–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications, including:
- Detection and analysis of PLP-specific effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
- Isolation of viable PLP-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of PLP-specific effector/memory T cells from naive T cell populations.
- Pulsing of antigen-presenting cells.
Overview
PepTivator® PLP is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the human PLP protein (UniProt ID: P60201).
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.
