Description
Background information
EBV is a human γ-herpesvirus with B cell growth–transforming ability and lymphomagenic potential. In healthy individuals, EBV typically establishes a persistent latent infection.
The immediate-early transcription factor BRLF1 controls the initiation of viral lytic gene expression and lytic reactivation from latency. It is an immunogenic target in EBV-associated malignancies.
Downstream applications
PepTivator EBV BRLF-1 – research grade has been specifically developed for efficient in vitro stimulation of EBV BRLF-1–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications, including:
- Detection and analysis of EBV BRLF-1-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
- Isolation of viable EBV BRLF-1-specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of EBV BRLF-1-specific CD4+ and CD8+ effector/memory T cells from naive T cell populations.
- Pulsing of antigen-presenting cells.
Overview
PepTivator® EBV BRLF-1 is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the complete sequence of the Epstein-Barr Virus BRLF-1 protein (UniProt ID: P03209).
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.
