Miltenyi, 130-099-023, CD271 MicroBead Kit, human

Background information
CD271, also known as LNGFR (low-affinity nerve growth factor receptor) or p75NTR, belongs to the low-affinity neurotrophin receptor and the tumor necrosis factor receptor superfamily.
CD271 is a well-known marker on mesenchymal stem cells, also known as mesenchymal stromal cells (MSCs), from bone marrow aspirate1–4 or lipoaspirate5. After separation colony-forming unit fibroblast (CFU-F) activity was found only in the CD271+ cell fraction, and not in the CD271– population6–8. Isolated CD271+ cells have a higher proliferative capacity in comparison to MSCs isolated by plastic adherence6,8,9. Secretion of growth factors was significantly higher in the separated CD271+ MSCs7.
CD271 (LNGFR) was initially described to be expressed on cells of the central and peripheral nervous system nervous system and was suggested to be involved in the development, survival, and differentiation of neural cells10. CD271+ neural crest progenitor cells can be efficiently isolated with Neural Crest Stem Cell MicroBeads.

Columns
MS, LS, XS, or autoMACS® Columns.

Overview
CD271 MicroBead Kits have been developed for the positive selection or depletion of cells expressing CD271 (LNGFR). They are ideally suited for the isolation of CD271+ mesenchymal stromal cells (MSCs) from human tissues, including bone marrow and lipoaspirate. In addition, CD271 MicroBead Kits have been used successfully for the isolation of MSCs from bone marrow of monkey, goat, dog, pig, and sheep.