Miltenyi, 130-099-138, ACSA-2 Antibody, anti-mouse, pure

Name: ACSA-2 Antibody, anti-mouse, pure
Brand: Miltenyi
SKU: 130-099-138

Size:100 µg in 1 mL, Mouse brain tissue postnatal day 6 was dissociated using the Neural Tissue Dissociation Kit (P) and the gentleMACS™ Dissociator. Mouse brain cells were stained with Anti-ACSA-2 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Catalog Number: 130-099-138

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Category

Antibody Labeling Kits

Pack Size100 µg

TagsMACS Antibodies Hybridomas

Description

Overview
The Anti-ACSA-2 antibodies (ACSA-2: astrocyte cell surface antigen-2) have been developed for the detection of astrocytes from cell suspensions of mouse neural tissue based on the expression of the ACSA-2 antigen.
The ACSA-2 antigen is specifically expressed on GLAST (ACSA-1) positive astrocytes and is therefore a specific marker of astrocytes in the developing and neonatal mouse central nervous system (CNS).
The percentage of ACSA-2 positive astrocytes differs according to the mouse age and the brain region. As the ACSA-2 antibodies are also detected by Fc-receptors expressed by CD11b+ microglia cells, the use of the FcR Blocking Reagent is strongly recommended

Specifications

General

Clone	IH3-18A3
Clonality	monoclonal
Isotype	rat IgG2b
Isotype control	Isotype Control Antibody, rat IgG2b
Host	rat
Type of antibody	Primary antibodies
Species reactivity	mouse
Antigen	ACSA-2
Distribution of antigen	CNS cells, brain
RRID	AB_2904626, AB_2651192, AB_2811488, AB_2727978, AB_2727930

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