Miltenyi, 130-099-895, Mouse LIF, premium grade

Biological activity

Background information
LIF is a pleiotropic cytokine, which is critically involved in embryonic development and blastocyst implantation. LIF belongs to the interleukin 6 family and functions through the gp130 activation of STAT3. In mice LIF is a key factor that prevents embryonic stem cells (ESC) to differentiate. Additionally, LIF affects hematopoiesis, neural development, bone and energy metabolism, and inflammation.

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Overview
LIF stands for leukemia inhibitory factor. Mouse LIF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.