Description
Background information
CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts, endothelial progenitor cells, and mature endothelial cells.
The CD34 MicroBead Kit UltraPure contains MicroBeads directly conjugated to CD34 antibodies for magnetic labeling of CD34-expressing cells from peripheral blood, cord blood, bone marrow, or apheresis harvest. Hematopoietic progenitors present at a frequency of 0.05-0.2% among peripheral blood mononuclear cells (PBMCs), 0.1-0.5% among cord blood mononuclear cells (MNCs) or 0.5-3% among bone marrow MNCs can be rapidly and efficiently enriched.
The CD34 MicroBead Kit UltraPure is suited for all routine CD34+ cell isolations. In addition, its unique formulation provides particular advantages with debris-rich sample material.
Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.
Downstream applications
- CD34+ cell expansion in culture1
- Lentiviral transduction1
- Molecular analysis of leukemic stem cells, e.g. AML blasts or CD34+ myelofibrosis samples2
- In vitro differentiation of CD34+ cells3,4
- Isolation of endothelial progenitor cells5
- Generation of humanized mice6
Columns
MS, LS, XS, or autoMACS® Columns.
Overview
The CD34 MicroBead Kit UltraPure is the latest development for positive selection of CD34+ hematopoietic stem and progenitor cells. It is suited for all routine CD34 cell isolations and is the ideal choice for debris-rich, low frequency samples.
