Miltenyi, 130-100-629, Monocyte Isolation Kit (BM), mouse

Detailed separation procedure
Using the Monocyte Isolation Kit (BM), mouse, monocytes are isolated by depletion of non-target cells. Non-target cells are indirectly magnetically labeled with a cocktail of biotin-conjugated monoclonal antibodies, as primary labeling reagent, and anti-biotin monoclonal antibodies conjugated to MicroBeads as secondary labeling reagent. The unwanted cells are depleted by retaining them within a MACS Column in the magnetic field of a MACS Separator, while the unlabeled monocytes pass through the column.

Downstream applications
Isolation of mouse monocytes from mouse bone marrow for in vitro analysis, differentiation studies, co-culture or adoptive transfer experiments.

Columns
LS or autoMACS® Columns.

Overview
The Monocyte Isolation Kit (BM) has been developed for the easy isolation of monocytes from mouse bone marrow by depletion of unwanted cells.