Miltenyi, 130-100-830, CD133 MicroBead Kit – Hematopoietic Tissue, human

Background information
The CD133 MicroBead Kit – Hematopoietic Tissue is a magnetic labeling system designed for the positive selection of CD133+ cells. It allows the single-step isolation of endothelial and hematopoietic stem and progenitor cells.
The CD133 molecule is a 5-transmembrane cell surface antigen with a molecular weight of 117 kDa. In the hematopoietic system, CD133 expression is restricted to a subset of CD34bright stem and progenitor cells in human fetal liver, bone marrow, cord blood, and peripheral blood. Isolated from hematopoietic sources, CD133+ cells can become adherent and are reported to become CD133– during culture. The CD34+CD133+ cell population, which includes CD34+CD38– cells, was shown to be capable of repopulating NOD/ SCID mice. In addition, the CD133 antigen is also expressed on circulating endothelial progenitor cells. The CD133 MicroBead Kit – Hematopoietic Tissue has been optimized for the isolation of CD133+ cells of hematopoietic or endothelial origin. For the isolation of CD133+ cells from solid tissues, e.g., the purification of cancer stem cells from solid tumors, we recommend the CD133 MicroBead Kit – Tumor Tissue (# 130-100-857).

Columns
For positive selection: MS, LS, XS, or autoMACS® Columns. For depletion: LD, D, or autoMACS Columns.

Overview
The CD133 MicroBead Kit - Hematopoietic Tissue has been developed for easy one-step isolation of CD133+ hematopoietic or endothelial stem and progenitor cells from hematopoietic tissues such as PBMCs, bone marrow or cord blood.