Miltenyi, 130-100-832, Anti-TRA-1-60 MicroBeads, human

Name: Anti-TRA-1-60 MicroBeads, human
Brand: Miltenyi
SKU: 130-100-832

Size:for up to 2×108 total cells

Catalog Number: 130-100-832

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Category

Human Primary Cells

TagsMACS Cell Separation Cell separation reagents MicroBeads and Isolation Kits ES and iPS cells

Description

Background information
The Anti-TRA-1-60 monoclonal antibody reacts with a pluripotent stem cell–specific antigen expressed on undifferentiated human embryonic stem (ES) cells, induced pluripotent (iPS) cells, embryonal carcinoma (EC) cells, and embryonic germ (EG) cells.2,3 The expression of TRA-1-60 on human ES cells is down-regulated upon differentiation. The Anti-TRA-1-60 antibody recognizes a neuraminidase-resistant carbohydrate epitope expressed on podocalyxin, a member of the CD34-related family of sialomucins. Podocalyxin is a transmembrane glycoprotein, which has been implicated in the development of aggressiveness in a variety of cancers including breast cancer and prostate cancer.

Applications
- Positive selection of undifferentiated TRA-1-60+ pluripotent stem cells, for example, human ES and iPS cells.
- Efficient enrichment of iPS cells during or after reprogramming1,5.

Columns
MS or autoMACS® Columns for use with Anti-TRA-1-60 MicroBeads.

Overview
The direct Anti-TRA-1-60 MicroBeads have been developed for the positive selection of human pluripotent stem cells based on the expression of the TRA-1-60 antigen.
The direct Anti-TRA-1-60 MicroBeads offer a 10 min isolation procedure, minimizing cell handling time.

Ordering

Notice

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