Miltenyi, 130-100-875, Pan Dendritic Cell Isolation Kit, mouse

Detailed separation procedure
Using the Pan Dendritic Cell Isolation Kit, mouse dendritic cells are isolated by depletion of non-target cells (negative selection). Non-target cells are labeled with a cocktail of biotin-conjugated monoclonal antibodies against antigens that are not expressed by dendritic cells. In a second step, cells are labeled with Anti-Biotin MicroBeads. The magnetically labeled non-target cells are depleted by retaining them within a MACS Column in the magnetic field of a MACS Separator while the unlabeled DCs run through.

Downstream applications
Dendritic cells isolated with the Pan Dendritic Cell Isolation Kit can be used for:
- Analysis of antigen uptake, processing, and presentation
- Stimulation of primary T cell responses by antigen-pulsed dendritic cells
- Elucidation of specific signal transduction pathways

Columns
LS or autoMACS® Columns.

Overview
The Pan Dendritic Cell Isolation Kit, mouse is a fast enrichment kit for untouched isolation of all dendritic cell subpopulations, both classical dendritic cells and plasmacytoid dendritic cells, from murine spleen.