Miltenyi, 130-101-372, Neonatal Cardiac Fibroblast Isolation Kit, mouse

Background information
Cardiac fibroblasts are one of the most frequent cell types within the heart and play a critical role (i) in heart development, (ii) in maintaining normal cardiac function, as well as (iii) in cardiac remodeling during pathological conditions such as myocardial infarction.The Neonatal Cardiac Fibroblast Isolation Kit, mouse has been designed for the enrichment of cardiac fibroblasts from dissociated neonatal mouse hearts. The isolation of cardiac fibroblasts has been successfully tested using neonatal mouse hearts from postnatal day 0 to day 3 (P0– P3). For optimal results, the Neonatal Cardiac Fibroblast Isolation Kit should be used in combination with the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373).

Applications
- Enrichment of cardiac fibroblasts from neonatal (P0-P3) mouse hearts.
- Culture and expansion or direct use of enriched cardiac fibroblasts for biochemical, physiological and pharmacological studies.

Columns
MS or autoMACS® Columns.

Overview
The Neonatal Cardiac Fibroblast Isolation Kit, mouse has been designed for the enrichment of cardiac fibroblasts from dissociated neonatal mouse hearts. The isolation of cardiac fibroblasts has been successfully tested using neonatal mouse hearts from postnatal day 0 to day 3 (P0– P3).