Miltenyi, 130-101-693, Mouse SCF, premium grade

Biological activity

Background information
Stem cell factor (SCF), also known as c-kit ligand, is a hematopoietic growth factor important for the survival, proliferation, and differentiation of hematopoietic stem cells and progenitor cells. Besides its pivotal role in mast cell development, SCF acts as a potent mast cell chemoattractant and upregulates mast cell adhesion and migration. SCF signals through the c-kit receptor (CD117) and exists in two forms, cell surface bound SCF and soluble SCF. The secreted soluble form of SCF is produced by the proteolytic processing of the cell surface anchored precursor molecule. Recombinant Mouse SCF corresponds to the secreted soluble form.

Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).

Overview
SCF stands for stem cell factor, also termed c-Kit ligand. Mouse SCF is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.