Miltenyi, 130-101-900, CD154 Antibody, anti-mouse, Biotin

Name: CD154 Antibody, anti-mouse, Biotin
Brand: Miltenyi
SKU: 130-101-900

Size:30 µg in 1 mL, Splenocytes from a KLH-immunized mouse were restimulated in vitro with or without KLH, CD28, and CD40 antibodies for six hours. Cells were harvested and either unstained or stained with CD154 antibodies and CD4 antibodies, and analyzed by flow cytometry. A lymphocyte gate based on forward and side scatter properties was activated. Dead cells and B cells were excluded according to PI and CD45R/B220-PerCP™ staining.

Catalog Number: 130-101-900

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Category

Biotin-Conjugated Secondary Antibodies

Pack Size30 µg

TagsMACS Antibodies Hybridomas

Description

Overview
Clone MR1 specifically recognizes the mouse CD154 antigen, a 39 kDa transmembrane glycoprotein also known as CD40L, gp39, T-BAM, TRAP, or Ly-62. CD154 is transiently up-regulated on activated CD4+ T cells and plays an important role as a costimulatory molecule in T cell/antigen-presenting cell interactions through ligation of CD40. The antibody has been shown to block the activation of antigen-presenting cells by T helper cells in vitro. Due to its transient expression within hours after activation, CD154 can be used as a marker for activated antigen-specific CD4+ T cells. Adding a CD40-blocking antibody during the stimulation of cell suspensions prevents the down-regulation of CD154. Blocking of CD40 is not required for intracellular detection of CD154 expression or if a pure population of enriched T cells is used.

Specifications

General

Clone	MR1
Clonality	monoclonal
Isotype	hamster IgG3κ
Host	hamster
Type of antibody	Primary antibodies
Species reactivity	mouse
Antigen	CD154
Molecular mass of antigen [kDa]	29
Distribution of antigen	dendritic cells, macrophages, mast cells, monocytes, NK cells, T cells, lymphocytes, basophils
Entrez Gene ID	21947
RRID	AB_2661126, AB_2661124, AB_2661125, AB_2661128, AB_2661127

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