Miltenyi, 130-103-464, Epidermis Dissociation Kit ACF, human

Background information
Human epidermal tissue can be dissociated to single-cell suspensions by combining mechanical dissociation with enzymatic degradation of the extracellular adhesion proteins, which maintain the structural integrity of tissues.
In a first step, the epidermal cell layer is removed from the dermal cell layer after enzymatic treatment over night at 4 °C. The epidermal tissue is then further digested enzymatically and dissociated into a single-cell suspension by using the gentleMACS™ Dissociators.

Overview
The Epidermis Dissociation Kit ACF, human enables the gentle and efficient generation of single-cell suspensions from human epidermal tissue. The kit has been particularly developed for the isolation of keratinocytes from diverse human skin biopsies.
This product is animal component-free (ACF) as it does not contain any primary raw materials derived directly from animals during the manufacturing process.