Miltenyi, 130-103-466, B-CLL Cell Isolation Kit, human

Detailed separation procedure
Non-B cells are indirectly magnetically labeled by using a cocktail of biotin-conjugated antibodies against CD2, CD3, CD4, CD14, CD15, CD16, CD56, CD61, CD235a, FcεRI, CD34, and Anti-Biotin MicroBeads. Isolation of untouched B cells is achieved by depletion of magnetically labeled cells. The depletion cocktail does not contain CD43, which might be expressed on the target cells.

Downstream applications
The B-CLL Cell Isolation Kit can be used for studies on B cells from tumor cell-containing samples and for functional studies on B cells. Furthermore the kit can be used for studies on signal requirements for B cell activation, induction of B cell proliferation, differentiation of B cells, induction of apoptosis in B cells, etc. Further applications include studies on signal transduction in B cells and the analysis of immunoglobulin class switching and somatic hypermutation in B cells.

Columns
MS, LS, or autoMACS® Columns.

Overview
The B-CLL Cell Isolation Kit was developed for the fast and efficient isolation of untouched B cells from PBMCs. The kit has been especially designed for the isolation of B cells from PBMCs of B-CLL or other B cell malignancies.
The B-CLL Cell Isolation Kit replaces the B Cell Isolation Kit (B-CLL), human.