Miltenyi, 130-103-925, StemMACS™ LDN-193189

Background information
StemMACS™ LDN-193189 inhibits the BMP type I receptors ALK2 (IC50 = 5 nM) and ALK3 (IC50 = 30 nM) and prevents phosphorylation of their downstream targets Smad1, Smad5 and Smad8. In contrast, LDN-193189 has little effect on ALK4, ALK5, and ALK7. LDN-193189 is a derivative of dorsomorphin, and has been used instead of the protein Noggin for inhibition of BMP signaling. In pluripotent stem cell research, LDN-103189 is used together with small molecules or growth factors to modulate stem cell differentiation, e.g., during neural induction or dopaminergic differentiation.

Applications
Published applications of LDN-193189 include:
- Differentiation of human ES and iPS cells into neural progenitors (in combination with SB431542)1
- Differentiation of human ES and iPS cells into neural crest cells2

Overview
StemMACS™ LDN-193189 is a selective and highly potent small molecule inhibitor of BMP signaling.