Description
Background information
The StemMACS MSC Expansion Media Kit XF is an optimized and standardized serum-free and xeno-free medium for the reproducible and reliable generation and expansion of mesenchymal stem cells (MSCs) from human bone marrow (BM) samples or other tissue sources. As MSCs appear at relatively low frequency in human BM samples and other tissues, their in vitro propagation is often necessary in order to obtain sufficient cell numbers for further experiments, such as in vivo transplantation studies in animals or in vitro differentiation or functional characterization. The formulation of StemMACS MSC Expansion Media Kit XF was designed to efficiently support the expansion of MSCs in vitro while maintaining their differentiation potential. The media formulation is xeno- and serum-free and is manufactured under strictly controlled conditions using ingredients of the highest quality. StemMACS Expansion Media Kit XF offers consistent lot-to-lot performance and optimal conditions for the cultivation of MSCs. It is suitable for the derivation, expansion, and enumeration using the colony-forming–unit fibroblast (CFU-F) assay and does not require the use of any cell attachment substrate for optimal performance.
Overview
The StemMACS MSC Expansion Medium Kit XF is an optimized and standardized serum- and xeno-free medium for the reproducible and reliable expansion and enumeration of MSCs from human BM samples or other tissue sources, while maintaining the cells' multi-lineage differentiation potential. StemMACS MSC Expansion Medium Kit XF is completely free of non-human animal derived components and does not require the use of cell attachment substrates.
Detailed procotols for MSC culture can be found on the Resource tab.
