Miltenyi, 130-104-183, Neonatal Cardiac Endothelial Cell Isolation Kit, mouse

Background information
Besides cardiomyocytes and fibroblasts, endothelial cells represent the third most frequent cell population in the neonatal mouse heart. Endothelial cells form the inner layer of blood vessels and play a key role in vascular development, homeostasis and remodeling. The Neonatal Cardiac Endothelial Cell Isolation Kit, mouse has been designed for enrichment of cardiac endothelial cells from dissociated neonatal mouse hearts. The isolation of cardiac endothelial cells has successfully been tested using neonatal mouse hearts from postnatal day 0 to day 3 (P0-P3). For optimal use, the Neonatal Cardiac Endothelial Cell Isolation Kit should be used in combination with the Neonatal Heart Dissociation Kit, mouse and rat (# 130-098-373).

Columns
For the first magnetic separation (depletion): LD or autoMACS® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.

Overview
The Neonatal Cardiac Endothelial Cell Isolation Kit, mouse has been developed for the two-step isolation of vital murine cardiac endothelial cells (P0-P3). Enriched cardiac endothelial cells are fully functional and can be used for various downstream applications.