Description
Background information
The transcription factor Nurr1 is a member of the steroid-thyroid hormone-retinoid receptor superfamily. Mutations in this gene have been associated with disorders related to dopaminergic dysfunction, including Parkinson disease, schizophrenia, and manic depression. Nurr1 induces the expression of tyrosine hydroxylase (TH), a critical factor for differentiation into dopaminergic neurons. In pluripotent stem cell research, Nurr1 has been used in combination with other transcription factors (e.g. Mash1, Lmx1a) to promote dopaminergic neuron differentiation.
Detailed procedure
For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.
Quality description
StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.
Overview
StemMACS™ Nurr1 (NR4a2) encodes the human transcription factor Nurr1 for transient transfection.
