Miltenyi, 130-104-453, Naive CD4+ T Cell Isolation Kit, mouse

Detailed separation procedure
Mouse naive CD4+ T cells are isolated by depletion of memory CD4+ T cells and non-CD4+ T cells. Single-cell suspensions of mouse spleen or lymph nodes are indirectly magnetically labeled with a cocktail of biotinylated monoclonal antibodies, as primary labeling reagent, and Anti-Biotin MicroBeads, as secondary labeling reagent. Simultaneously, CD44 MicroBeads are added to label memory T cells. No washing steps are required during magnetic labeling. The magnetically labeled non-target cells are depleted by retaining them on a MACS® Column in the magnetic field of a MACS Separator, while the unlabeled target cells pass through the column.

Applications
Naïve CD4+ T cells are perfect for use in in vitro analysis of T cell differentiation, adoptive transfer experiments, studies on signal requirements for CD4+ T cell activation and proliferation, and for studies on regulation of CD4+ T cell cytokine expression.

Columns
LS or autoMACS® Columns.

Overview
The Naive CD4+ T Cell Isolation Kit, mouse has been developed for the isolation of untouched naive CD4+ T helper cells from suspensions of mouse spleen cells or lymph node cells.