Description
Biological activity
Background information
Fibroblast growth factor 2 (FGF-2), also termed fibroblast growth factor basic (FGF-b) or basic FGF (bFGF), belongs to the FGF family. It functions as a wide-spectrum mitogenic, angiogenic, and neurotrophic factor and stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal, and endothelial cells. FGF-2 has been implicated in a multitude of physiological and pathological processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF-2 IS is an engineered FGF-2 variant with increased thermostability and higher resistance to proteases, and retains the same biological properties as naturally occurring FGF-2.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).
Overview
FGF-2 IS stands for fibroblast growth factor 2 "Improved Sequence", also termed fibroblast growth factor basic (FGF-b) or basic FGF. Human FGF-2 IS is a variant of Human FGF-2 with a proprietary amino acid substitution. Human FGF-2 IS is covered by patent nos. US10,336,799 and EP2930181B1. Human FGF-2 IS is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
