Description
Background information
The StraightFrom® Whole Blood Anti-ROR1 MicroBead Kit has been developed for the positive selection of ROR1+ cells directly from anticoagulated whole blood by using the autoMACS® Pro Separator or the Whole Blood Column Kit. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis. ROR1 has characteristics of an oncofetal gene and was recently identified to be overexpressed at the gene and protein levels in chronic lymphocytic leukemia (CLL). ROR1 expression has also been detected in ovarian cancer, renal cancer, melanoma, and lung adenocarcinoma, suggesting a general role of ROR1 in cancer genesis and/or maintenance. Moreover, to a much lower level ROR1 expression has been found in undifferentiated embryonic stem cells, in adipose tissue, in early stage of B cell development, but not in major adult tissues. In blood samples with low frequencies of CLL B cells, e.g., in minimal residual disease, the enrichment of these cells via the StraightFrom Whole Blood Anti-ROR1 MicroBead Kit allows analysis and downstream processing of these cells.
Detailed separation procedure
First, the ROR1+ cells in a whole blood sample are indirectly magnetically labeled with StraightFrom® Whole Blood Anti-ROR1-PE and Anti-PE MicroBeads. Then, the cell suspension is loaded onto a MACS® Column, which is placed in the magnetic field of a MACS Separator. The magnetically labeled ROR1+ cells are retained within the column. The unlabeled cells run through; this cell fraction is thus depleted of ROR1+ cells. After removing the column from the magnetic field, the magnetically retained ROR1+ cells can be eluted as the positively selected cell fraction.
Columns
autoMACS® Columns or Whole Blood Columns.
Overview
The StraightFrom® Whole Blood Anti-ROR1 MicroBead Kit was developed for rapid positive selection of ROR1+ cells directly from human whole blood. No sample preparation is required, including density gradient centrifugation or erythrocyte lysis.
