Miltenyi, 130-105-760, GM-CSF Secretion Assay – Cell Enrichment and Detection Kit (PE), human

Background information
Granulocyte macrophage colony-stimulating factor (GM-CSF) is a hematopoietic growth factor that is essential for proliferation and development of granulocyte and monocyte/macrophage progenitors. It also functions as a growth factor for erythroid and megakaryocytic precursor cells in conjunction with erythropoietin. GM-CSF is secreted by various cell types including T cells, macrophages, endothelial cells, and fibroblasts in response to inflammatory stimuli and cytokines. In addition, GM-CSF strongly chemoattracts neutrophils and eosinophils and enhances the effector functions of neutrophils and macrophages.

Downstream applications
- Enrichment and detection of viable GM-CSF–secreting leukocytes.
- Enrichment and detection of viable GM-CSF–secreting leukocytes from whole blood.
- Enrichment and detection of GM-CSF–secreting, antigen-specific T cells for enumeration and phenotypic characterization as well as for expansion and functional characterization.
- Monitoring and analysis of very low frequent antigen-specific T cell immunity in autoimmunity.
- Isolation and expansion of antigen-specific T cells.
- Enrichment and analysis of GM-CSF–secreting cells for determination of functional antigens in disease and for T cell receptor (TCR) epitope mapping.
- Analysis or cloning of TCR repertoire of antigen-specific T cells.

Columns
MS, LS, or autoMACS® Columns.

Overview
The GM-CSF Secretion Assay - Cell Enrichment and Detection Kit was developed for enrichment and sensitive detection of human GM-CSF-secreting cells.