Miltenyi, 130-106-639, Adipose Tissue Progenitor Isolation Kit, mouse

Background information
In mammals the two main types of adipose tissues exist, white adipose tissue (WAT) and brown adipose tissue (BAT). Both types fulfil different physiological functions. White adipose tissue (WAT) is for storing energy, while brown adipose tissue (BAT) is for energy consumption. Using the Adipose Tissue Progenitor Isolation Kit, mouse adipose tissue derived progenitor cells are isolated by depletion of non-target cells followed by positive selection of the target cells. Non-target cells are directly magnetically labeled with a cocktail of monoclonal antibodies conjugated with MACS MicroBeads. The magnetically labeled non-target cells are depleted by retaining them within a MACS Column in the magnetic field of a MACS Separator, while the unlabeled adipose tissue progenitor cells pass through the column. Subsequently, the progenitor cells are directly magnetically labeled. The magnetically labeled target cells are retained within a MACS Column in the magnetic field of a MACS Separator, while the unlabeled non-target cells pass through the column.

Overview
The Adipocyte Progenitor Isolation Kit, mouse has been designed for the isolation of tissue-derived progenitor cells from dissociated brown and white adipose tissue. For optimal results, the Adipocyte Progenitor Isolation Kit, mouse should be used in combination with the Adipose Tissue Dissociation Kit, mouse and rat (# 130‑105‑808).