Miltenyi, 130-106-769, StemMACS™ Flp Recombinase mRNA

Name: StemMACS™ Flp Recombinase mRNA
Brand: Miltenyi
SKU: 130-106-769

Size:20 µg

Catalog Number: 130-106-769

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Category

mRNA Isolation Kits

Pack Size20 µg

TagsMACS Cell Culture and Stimulation mRNA transfection

Description

Background information
Flippase (Flp) is a site-specific recombinase derived from the yeast Saccharomyces cerevisiae. It mediates recombination between two identical flippase recognition target (FRT) sites.

Detailed procedure
For satisfactory transfection results, use a transfection protocol optimized for your specific cell type. StemMACS™ eGFP mRNA or StemMACS™ Nuclear eGFP mRNA allow easy evaluation of transfection efficiency and are recommended as positive controls.

Quality description
StemMACS™ mRNAs are highly pure, in vitro-transcribed mRNAs that have been carefully optimized to ensure high level expression after transfection. Polyadenylation and enzymatic cap mimic a fully processed mature mRNA and ensure efficient translation. Incorporation of the modified nucleotides pseudouridine and 5-methylcytidine reduces the innate antiviral response to single-stranded mRNA. Robust expression of the encoded factor after transfection has been verified by immunofluorescence or flow cytometry.
StemMACS™ mRNAs are lyophilized from a 0.2 µM filtered solution for extended shelf-life.

Overview
StemMACS™ Flp Recombinase mRNA encodes a codon optimized version of Flp recombinase.

Ordering

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