Description
Background information
TLR9 is a prominent member of the toll-like-receptor (TLR) family recognizing pathogen-associated molecular patterns. TLR9 recognizes specifically unmethylated CpG motifs in bacterial DNA leading to activation of immune cells1,2. These effects can be mimicked by short synthetical oligodeoxyribonucleotides (ODNs) containing unmethylated CpG motifs3.
Several classes of CpG ODN have been identified and can be distinguished by their effects on certain cell types. A-class ODNs containing 5’ and 3’ G-rich stretches induce high levels of type I IFN but show low activation of B cell proliferation. B-class ODNs activate B cells and TLR9-dependent NFkB signalling in recombinant cell lines but show low induction of IFN-α. C-class ODN induce high amounts of IFN-α and activate B cells. The recently discovered new P-Class ODNs show similar but superior properties to C-class ODNs.
ODN 1826 is a B-class ODN that strongly activates murine TLR9.
Applications
CpG ODNs can be used for activation of immune cells, such as human peripheral blood mononuclear cells (PBMCs), murine splenocytes, or isolated immune cells (e.g., B cells and pDCs). CpG ODNs can be used to activate signaling in TLR9- expressing recombinant cell lines.
Overview
Oligodeoxyribonucleotides containing CpG motifs (CpG ODNs) are widely used for activation of immune cells, such as human PBMCs, murine splenocytes or isolated immune cells, e.g., B cells and pDCs. They also activate signalling in TLR9-expressing recombinant cell lines.
ODN 1826 strongly activates murine TLR9. It is a B-class ODN for activation of B cells, induction of Il-6 secretion, and activation of NF-kB-signalling pathways.
