Description
Biological activity
Background information
Fibroblast growth factor 4 (FGF-4) is a heparin-binding growth factor and member of the FGF family, which controls several physiological and pathological development processes, including cell growth, cell survival, and morphogenesis. FGF-4 is expressed during embryo development and in several tissues during the adult life, and binds both receptors FGFR 1c, 2c, 3c, and 4. FGF-4 function partially overlaps with the one of FGF-8. By promoting cell differentiation, FGF-4 plays a central role during prenatal development and tissue regeneration, but can also have a pathological role as oncogene in tumor growth. Increased levels of FGF-4 was found in several human tumors. Altered levels of FGF-4 during embryo development can result in abnormal limb formation. Mature human FGF-4 shares 86% amino acid identity with mouse FGF-4.
Quality description
Research-grade cytokines are suitable for a wide variety of cell culture applications. They are sterile-filtered prior to lyophilization. Generally, endotoxin levels are <0.1 ng/μg (<1 EU/μg), and purities are >95%. The biological activity is tested in appropriate bioassays.
Premium-grade cytokines offer the convenience of high and well-defined biological activities and allow exact unit dosing for demanding applications. The biological activity is determined after lyophilization and reconstitution, and normalized to WHO/NIBSC standards whenever available. In general, endotoxin levels are <0.01 ng/μg (<0.1 EU/μg), and purities are >97%. Lot-specific activities are stated in the Certificate of Analysis (www. miltenyibiotec.com/certificates).
Overview
FGF-4 stands for fibroblast growth factor 4. Human FGF-4 is a recombinant protein optimized for use in cell culture, differentiation studies, and functional assays.
