Miltenyi, 130-110-187, Tumor Cell Isolation Kit, mouse

The Tumor Cell Isolation Kit, mouse has been designed for the enrichment of untouched mouse tumor cells from mouse tumors.
During the growth phase in vivo, tumor tissue is vascularized and infiltrated by cells of non-tumor origin, including heterogeneous lymphocyte subpopulations, fibroblasts, and endothelial cells.
The level of infiltration is highly dependent on multiple factors like tumor subtype, growth rate, site of tumor growth and status of the host animal. However, even when these factors are kept constant, the amount and composition of infiltrating cells remain widely unpredictable. Molecular downstream analysis as well as culture of mouse tumor cells is frequently challenged by non-tumor cells. Additionally, there is a lack of markers exclusively expressed on mouse tumor cells, which makes direct isolation or identification in analyses like flow cytometry difficult.
For optimal results, the Tumor Cell Isolation Kit, mouse should be used in combination with the Tumor Dissociation Kit, mouse (# 130-096-730) and gentleMACS™ Dissociators.

Columns
LS Columns, autoMACS® Columns, or Multi-24 Column Blocks.

Overview
The Tumor Cell Isolation Kit, mouse enables the enrichment of mouse tumor cells from primary mouse specimens using MACS® Cell Separation technology.
While depleting tumor associated cells such as lymphocyte subpopulations, fibroblasts, and endothelial cells in a fast and easy protocol, the tumor cells remain untouched, improving any downstream analysis.