Miltenyi, 130-110-434, Macrophage Isolation Kit (Peritoneum), mouse

Using the Macrophage Isolation Kit (Peritoneum), mouse, macrophages are isolated by depletion of non-target cells. Non-target cells are indirectly magnetically labeled with a cocktail of biotin-conjugated monoclonal antibodies, as primary labeling reagent, and anti-biotin monoclonal antibodies conjugated to MicroBeads as secondary labeling reagent. The magnetically labeled non-target cells are depleted by retaining them within a MACS Column in the magnetic field of a MACS Separator, while the unlabeled macrophages pass through the column.

Downstream applications
Isolation of mouse macrophages from peritoneal cavity of thioglycollate stimulated mice for in vitro analysis, differentiation studies, co-culture or adoptive transfer experiments.

Columns
MS, LS, or autoMACS® Columns.

Overview
The Macrophage Isolation Kit (Peritoneum) has been developed for the easy isolation of macrophages from peritoneal cavity of thioglycollate stimulated mice by depletion of unwanted cells.