Miltenyi, 130-111-569, Hoechst 33342 Staining Solution

Background information
Hoechst 33342 is a cell permeable nucleic acid stain that binds to minor groove A-T rich regions of double-stranded DNA. Therefore, the staining intensity differs between different cell types. All nucleated cells are Hoechst positive.
The fluorescence emission maximum for DNA-bound Hoechst 33342 is about 461 nm. With an excitation maximum of 355 nm it can be excited by a ultraviolet (UV) laser as well as by a violet laser (405 nm) and detected within the blue fluorescence channel, e.g., channel V1 on the MACSQuant® Analyzer 10.

Overview
The Hoechst 33342 Staining Solution is a ready-to-use reagent for the identification of nucleated cells by flow cytometric analysis. Hoechst 33342 is widely used for enumeration of nucleated cells and for determination of DNA content in cell cycle analysis.
Hoechst 33342 is also frequently used as a nuclear counterstain in fluorescence microscopy assays.