Miltenyi, 130-113-969, CD127 Antibody, anti-human, APC

Name: CD127 Antibody, anti-human, APC
Brand: Miltenyi
SKU: 130-113-969

Size:30 tests in 60 µL, Human peripheral blood mononuclear cells (PBMCs) were stained with CD127 antibodies as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. For all other conjugates the FcR Blocking Reagent has been used to avoid Fc receptor–mediated antibody labeling. Cell debris and dead cells were excluded from the analysis based on scatter

Catalog Number: 130-113-969

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Category

Antibody Labeling Kits

Pack Size30 tests

TagsMACS Antibodies Hybridomas

Description

Overview
Clone MB15-18C9 recognizes the CD127 antigen, which is the α-chain of the interleukin 7 (IL-7) receptor, a type I membrane glycoprotein. Signaling of IL-7 through the IL-7R requires both IL-7Rα and the common cytokine gamma chain (γc). CD127 can be identified on immature B cells through the early pre–B stage, on thymocytes, and on most mature T cells with transient down-regulation upon activation. On regulatory T cells CD127 is absent and its expression is inversely correlated with FoxP3 expression and suppressive function. CD127 is also used by thymic stromal derived lymphopoietin (TSLP) as part of a complex.

Specifications

General

Clone	MB15-18C9
Clonality	monoclonal
Isotype	mouse IgG2aκ
Isotype control	Isotype Control Antibody, mouse IgG2a
Host	mouse
Type of antibody	Primary antibodies
Species reactivity	human, non-human primate
Cross-reactivity	rhesus monkey (Macaca mulatta), cynomolgus monkey (Macaca fascicularis)
Antigen	CD127
Molecular mass of antigen [kDa]	49
Distribution of antigen	B cells, T cells, monocytes, lymphocytes, thymocytes, bone marrow, liver
Entrez Gene ID	3575
RRID	AB_2922081, AB_10829748, AB_2726160, AB_2733500, AB_2726437, AB_2733760, AB_2733336, AB_2732957, AB_2733501, AB_2733761, AB_2733337, AB_2922048, AB_2726159, AB_2732956, AB_2726436

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