Miltenyi, 130-114-822, CD34+CD38- Cell Isolation Kit, human

Background information
The CD34+CD38– Cell Isolation Kit has been developed for the isolation of the more primitive CD38– subpopulation of CD34+ cells. CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts, endothelial progenitor cells, and mature endothelial cells. CD38 is absent on most primitive CD34+ stem cells and upregulated on CD34+ cells upon lymphocyte commitment. CD34+CD38- cells are isolated in a two-step isolation protocol. First, the CD34+ cells are positively enriched using CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34+ are then labeled with CD38 MicroBeads to deplete CD38+ cells in a second separation step.

Downstream applications
- CD34+ CD38– cell expansion in culture
- Lentiviral transduction
- Molecular analysis
- In vitro differentiation of CD34+ CD38– cells
- Generation of humanized mice

Columns
LS Columns.

Overview
The CD34+CD38– Cell Isolation Kit, human has been developed to isolate a CD38– subset of CD34+ cells, i.e., primitive hematopoietic stem and progenitor cells, from cord blood, bone marrow, or apheresis.