Description
Background information
The Zika glycoprotein M domain from virus isolate Z1106033 polyprotein gene comprises the amino acids 123–294 of the full length polyprotein. The Zika virus belongs to the family of flaviviruses and is spread by Aedes mosquitos. In most cases no symptoms are observed after infection, but about 20% of Zika infected individuals react with symptoms like fever, red eyes, joint pain, and headache. During pregnancy the virus can spread to the fetus and cause microcephaly and other brain malformations. The virus is distributed in around 40 countries and the Aedes mosquitos can be found in all tropical and subtropical areas.
Downstream applications
PepTivator Zika Glycoprotein M – research grade has been specifically developed for efficient in vitro stimulation of Zika Glycoprotein M–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4+ and CD8+ T cells in various applications, including:
- Detection and analysis of antigen-specific CD4+ and CD8+ effector/memory T cells in PBMCs by MACS® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
- Isolation of viable antigen–specific CD4+ T cells with the CD154 MicroBead Kit, or of CD4+ and CD8+ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
- Generation of antigen–specific effector/memory T cells from naive T cell populations.
- Pulsing of antigen-presenting cells.
Overview
PepTivator® Zika Glycoprotein M is a pool of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap, covering the sequence of the Zika membrane glycoprotein precursor M from virus isolate Z1106033 polyprotein gene (GenBank Acc. no. KU312312.1).
In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.
