Description
Background information
HHV-1, also known as HSV-1, belongs to the family of Herpesviridae which are widespread among humans. Once infected, the virus persists for life. The Herpesviridae are not only known to cause various diseases like Herpes labialis or Herpes genitalis, but also life threatening complications in immunocompromized individuals (e.g. HHV-induced encephalitis).
Applications
Detection and analysis of antigen-specific CD4+ and CD8+ effector/memory T cells, for example, in peripheral blood mononuclear cells (PBMCs), by MACSR Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
● Isolation of viable antigen-specific CD4+ T cells with the CD154 MicroBead Kit.
● Isolation of viable antigen-specific CD4+ and CD8+ T cells using MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits or the CD137 MicroBead Kit for in vitro generation of T cell lines/clones for research.
● Generation of antigen-specific CD4+ and CD8+ effector/ memory T cells from naive T cell populations for research on immunotherapy and vaccination.
● Pulsing of antigen-presenting cells for research on dendritic cell vaccination.
Overview
PepTivator® HHV1 is a peptice pool that consists mainly of 15-mer sequences with 11 amino acids (aa) overlap, covering the complete sequence of the human herpes simplex virus 1 (HHV1) envelope glycoprotein D (UniProtKB Acc. no. P57083).
The PepTivator Peptide Pools have been specially developed for efficient in vitro stimulation of antigen-specific CD4+ and CD8+ T cells, as peptides of 15 amino acid length with 11 amino acid overlap represent the optimized solution for stimulating both CD4+ and CD8+ T cells in various applications. Stimulation of T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and upregulation of activation markers, which then allows the detection or isolation of antigen-specific T cells. Quantitative, henotypical, or functional analysis of antigen-specific T cell immunity can provide important information on the natural course of immune responses in healthy or immunocompromised individuals.
