Miltenyi, 130-115-169, MACSprep™ PBMC Isolation Kit, human

Background information
The isolation of human peripheral blood mononuclear cells (PBMCs) is performed with only one labeling step and in a two-step separation procedure. During the first isolation step erythrocytes are aggregated and sedimented. In a second step, PBMCs are isolated by depletion of non-PBMCs (e.g. neutrophils, eosinophils, platelets, and residual erythrocytes). Non-PBMCs are indirectly magnetically labeled with a cocktail of biotin-conjugated monoclonal antibodies and MACSprep™ Anti-Biotin MicroBeads. The magnetically labeled non-PBMCs are depleted by retaining them within a MACS Column in the magnetic field of a MACS® Separator while the unlabeled PBMCs run through.

Downstream applications
The MACSprep™ PBMC Isolation Kit, human is used for the fast isolation of human peripheral blood mononuclear cells (PBMCs).
Applications include:
- Efficient, fast, and convenient isolation of untouched PBMCs from blood samples of 1–8 mL
- PBMC co-cultures, e.g., functional immune cell assays
- Studies on cytokine expression of immune cells upon restimulation
- Studies on signal transduction during activation of immune cells
- Immune monitoring of antigen-specific T cells

Overview
The MACSprep™ PBMC Isolation Kit, human has been developed for the fast isolation of human peripheral blood mononuclear cells (PBMCs) from 1–8 mL of freshly drawn anticoagulated whole blood without density gradient centrifugation.