Miltenyi, 130-115-389, Neuron Isolation Kit, mouse

Background information
The Neuron Isolation Kit, mouse is an indirect magnetic labeling system for the isolation of untouched neurons from cell suspensions of mouse neural tissue. Non-neuronal cells like astrocytes, oligodendrocytes, microglia, endothelial cells, fibroblasts, except erythrocytes, are indirectly magnetically labeled by using biotin-conjugated antibodies specific for non-neuronal cells in combination with Anti-Biotin MicroBeads. Isolation of highly pure unlabeled neuronal cells is achieved by depletion of the magnetically labeled cells. The cell number and composition of the neuronal cell fraction differs according to the mouse age and the brain region used for cell isolation. The isolation of neurons has been tested with CD-1® mice aged from embryonic day 18 (E18) to adult. For optimal results, the Neural Tissue Dissociation Kit – Postnatal Neurons or the Adult Brain Dissociation Kit have been used prior to this kit.

Columns
LS or autoMACS® Columns.

Overview
The Neuron Isolation Kit, mouse depletes all non-neuronal cells so that "untouched neurons" remain.