Miltenyi, 130-115-660, StemMACS™ Trilineage Differentiation Kit, human

Background information
Pluripotency is the ability to differentiate into the three embryonal germ layers, ectoderm, mesoderm, and endoderm, and is a defining characteristic of pluripotent stem cells (PSCs). Therefore, basic characterization of PSC lines includes typically a test for pluripotency in addition to surface marker expression and morphology. However, traditional pluripotency assays such as embryoid body and teratoma formation are both time-consuming and difficult to quantitate.
The StemMACS Trilineage Differentiation Kit provides a functional assay that can be completed in 7 days. In contrast to other pluripotency assays, the StemMACS Trilineage Differentiation Kit allows either analysis by immunocytochemistry or quantitative analysis by flow cytometry. The kit is enabling parallel assessment of multiple hPSC lines. The 12-well format is optimal for flow cytometric analysis. For immunocytochemistry, the 24-well format may be sufficient.

Applications
Assessment of differentiation potential of human pluripotent stem cells.

Overview
The StemMACS Trilineage Differentiation Kit enables directed differentiation of pluripotent stem cells into ecto-, meso- and endoderm. Combined with immunofluorescent or flow cytometric analysis it provides a simple 7-day assay for functional characterization of ES and iPS cell lines.