Miltenyi, 130-115-818, NK Cell Isolation Kit, mouse

Downstream applications
NK cells isolated using the NK Cell Isolation Kit can be used for in vitro and in vivo applications, e.g., functional studies or adoptive transfer experiments.

Columns
MS, LS, or autoMACS® Columns.

Overview
The NK Cell Isolation Kit was developed for the isolation of untouched NK cells from single-cell suspensions of murine spleen. Non-NK cells, i.e. T cells, dendritic cells, B cells, granulocytes, macrophages, and erythroid cells are magnetically labeled by using a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. Isolation of highly pure unlabeled NK cells is achieved by depletion of non-target cells. The kit has been optimized to give outstanding purities in C57BL/6J mice.