Miltenyi, 130-116-474, Tumor-Associated Fibroblast Isolation Kit, mouse

Background information
The Tumor-Associated Fibroblast Isolation Kit, mouse enables the isolation of tissue-derived, tumor-associated fibroblasts from dissociated mouse tumors.
Tumor-associated fibroblasts have been shown to play an important role in tumor development, progression, and function. However, they may only make up about 0.5 – 5% of the total number of cells found in tumors. Applying a two-step strategy, tumor-associated fibroblasts are first pre-enriched by removing non-target cells, followed by isolation using the general fibroblast marker CD90.2. This allows reliable isolation from different syngeneic mouse tumor models.
For optimal results, the Tumor-Associated Fibroblast Isolation Kit, mouse should be used in combination with the Tumor Dissociation Kit, mouse (# 130-096-730) and the gentleMACS™ Dissociators.

Columns
MS, LD, or autoMACS® Columns.

Overview
The Tumor-Associated Fibroblast Isolation Kit, mouse has been designed for the isolation of tissue-derived, tumor-associated fibroblasts from dissociated mouse tumors.