Description
Background information
The CD8a antigen is expressed on most thymocytes, almost all cytotoxic T cells, and on subpopulations of dendritic cells. CD8a functions as an accessory molecule in the recognition of MHC class I/peptide complexes by the TCR heterodimer on cytotoxic CD8a+ T cells.
Downstream applications
CD8a (Ly-2) MicroBeads, mouse were developed for the isolation of mouse CD8+ T cells from various lymphoid and non-lymphoid tissues or from peripheral blood for the subsequent functional and phenotypical analysis of T cells in vitro and in vivo.
Examples for downstream applications include:
- In vitro and in vivo studies on protective immune responses against parasites or allergens
- Adoptive transfer into immunodeficient and virus-infected mice
- Isolation of highly pure CD8+ T cells from CNS of MHV-infected mice for evaluation of their chemokine expression patterns
- In vitro activation and expansion of CD8+ T cells (e.g. with the T Cell Activation/Expansion Kit, mouse)
- Antigen-specific stimulation of CD8+ T cells (e.g. with PepTivator® Peptide Pools)
- Analysis of cytokine secretion and surface marker expression (e.g. with REAfinity™ Recombinant Antibodies)
- Examination of the regulatory role of cytokines in autoimmune disease and infection
Columns
For positive selection: MS, LS, or autoMACS® Columns. For depletion: LD or autoMACS Columns.
Overview
Mouse CD8a (Ly-2) MicroBeads have been developed for positive selection or depletion of mouse CD8a+ T cells from single-cell suspensions of lymphoid and non-lymphoid tissues or from peripheral blood.
